کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1204537 1493645 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modeling of ion exchange expanded-bed chromatography for the purification of C-phycocyanin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Modeling of ion exchange expanded-bed chromatography for the purification of C-phycocyanin
چکیده انگلیسی

This work is focused on the experimental evaluation and mathematical modeling of ion exchange expanded-bed chromatography for the purification of C-phycocyanin from crude fermentative broth containing Spirulina platensis cells. Experiments were carried out in different expansion degree to evaluate the process performance. The experimental breakthrough curves were used to estimate the mass transfer and kinetics parameters of the proposed model, using the Particle Swarm Optimization algorithm (PSO). The proposed model satisfactorily fitted the experimental data. The results from the model application pointed out that the increase in the initial bed height does not influence the process efficiency, however enables the operation of expanded-bed column at high volumetric flow rates, improving the productivity. It was also shown that the use of mathematical modeling was a good and promising tool for the optimization of chromatographic processes.


► Mathematical modeling of the C-phycocyanin adsorption in expanded-bed columns.
► The proposed model satisfactorily fitted the experimental data.
► The increase in the initial resin height did not influence the process efficiency.
► Mathematical modeling is a promising tool to optimize chromatographic processes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1281, 15 March 2013, Pages 73–78
نویسندگان
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