کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1204558 965158 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of an anion-exchange hollow-fiber membrane adsorber containing γ-ray grafted glycidyl methacrylate chains
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Evaluation of an anion-exchange hollow-fiber membrane adsorber containing γ-ray grafted glycidyl methacrylate chains
چکیده انگلیسی

It is widely recognized that membrane adsorbers are powerful tools for the purification of biopharmaceutical protein products and for this reason a novel hollow-fiber AEX type membrane adsorber has been developed. The membrane is characterized by grafted chains including DEA ligands affixed to the pore surfaces of the membrane. In order to estimate the membrane performance, (1) dynamic binding capacities for pure BSA and DNA over a range of solution conductivity and pH, (2) virus reduction by flow-through process, and (3) HCP and DNA removal from cell culture, are evaluated and compared with several other anion-exchange membranes. The novel hollow-fiber membrane is tolerant of high salt concentration when adsorbing BSA and DNA. When challenged with a solution containing IgG the membrane has high impurity removal further indicating this hollow-fiber based membrane adsorber is an effective tool for purification of biopharmaceutical protein products including IgG.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1218, Issue 17, 29 April 2011, Pages 2381–2388
نویسندگان
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