کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1204754 965166 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of sildenafil, tadalafil and vardenafil by gas chromatography–mass spectrometry on short capillary column
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Identification of sildenafil, tadalafil and vardenafil by gas chromatography–mass spectrometry on short capillary column
چکیده انگلیسی

Sildenafil and its analogues (tadalafil and vardenafil) are phosphodiesterase type 5 inhibitors used in the treatment of male erectile dysfunction. Some dietary supplements, herbal preparations and food products which claim to enhance male sexual function have been found to be adulterated with these drugs. In this study, a gas chromatograph–mass spectrometer (GC–MS) assay was developed for identification of the drugs. In addition to good and short chromatographic separation that can be achieved within 6 min by using a short 10 m capillary column, no prior sample clean-up before GC–MS analysis was required, thus making this assay a cost saving and rapid method. Furthermore, the assay is specific as the identification of sildenafil, tadalafil and vardenafil were done by detection of molecular ions; m/z 474, 389 and 448, respectively, and several other characteristic ions resulted from the mass fragmentation of individual molecules. Using our currently developed assay, sildenafil and its analogues were successfully identified in food and herbal matrices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 47, 20 November 2009, Pages 8426–8430
نویسندگان
, , , ,