کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205078 965180 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimized use of a 50 μm ID secondary column in comprehensive two-dimensional gas chromatography–mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Optimized use of a 50 μm ID secondary column in comprehensive two-dimensional gas chromatography–mass spectrometry
چکیده انگلیسی

The objective of the present research is directed towards the optimized use of a 50 μm ID secondary column, in a comprehensive two-dimensional gas chromatography–quadrupole mass spectrometry (GC × GC–qMS) system. The analytical aim was achieved by exploiting a split-flow GC × GC approach, and a rapid-scanning qMS instrument. The stationary phase combination consisted of an apolar (silphenylene polymer) 30 m × 0.25 mm ID column, linked by means of a Y-union, to an MS-connected 1 m × 0.05 mm ID polar one [poly(ethyleneglycol)], and to a 0.20 m × 0.05 mm ID uncoated capillary segment; the latter was connected to a manually operated split-valve. It will be herein demonstrated that the split-flow GC × GC approach, successfully employed in previous H2-based, flame ionization detection experiments, provides equally satisfactory results using mass spectrometric detection and helium as carrier gas. An optimized split-flow GC × GC–qMS method was developed and exploited for the analysis of a perfume sample. The results attained were compared with those observed using the same analytical column combination, but with no flow-splitting. It was found that it is not convenient to employ a 50 μm ID secondary column in a conventional GC × GC–MS instrument. On the contrary, the use a 50 μm ID secondary column, in a split-flow, twin-oven system, provided a good performance. A recently developed comprehensive chromatography software was used for data processing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1217, Issue 25, 18 June 2010, Pages 4160–4166
نویسندگان
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