کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205206 965186 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
T cell activation on a single-cell level in dielectrophoresis-based microfluidic devices
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
T cell activation on a single-cell level in dielectrophoresis-based microfluidic devices
چکیده انگلیسی

The gentle and careful in vitro processing of live cells is essential in order to make them available to future therapeutic applications. We present a protocol for the activation of single-T cells based on the contact formation with individual anti-CD3/anti-CD28 presenting microbeads in a lab-on-chip environment. The chips consist of microfluidic channels and microelectrodes for performing dielectrophoretic manipulation employing a.c. electric fields. The dielectrophoretic guiding elements allow the assembly of cell–bead pairs while avoiding ill-defined physical contacts with their environment. After overnight cultivation of the manipulated cells, 77% of the bead-associated T cells expressed the activation marker molecule CD69. Physiological stress on the cells was shown to be mainly due to the single-cell cultivation and not to the manipulation in the chips. The same approach could be useful for the in vitro regulation of stem cell differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1202, Issue 1, 15 August 2008, Pages 83–89
نویسندگان
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