A generalization of a chromatographic response function for application in non-target one- and two-dimensional chromatography of complex samples

► CRF as a tool for optimization of different 1D and 2D chromatographic scenarios. ► The time-saving issue for 1D and 2D chromatography has been dealt with. ► A flexible and weighted CRF is presented for non-target chromatography. ► The CRF was successfully applied for several 1D and 2D chromatography scenarios.