Particle size analyses of porous silica and hybrid silica chromatographic support particles: Comparison of flow/hyperlayer field-flow fractionation with scanning electron microscopy, electrical sensing zone, and static light scattering

Porous silica and hybrid silica chromatographic support particles having particle diameters ranging approximately from 1 μm to 15 μm have been characterized by flow/hyperlayer field-flow fractionation (FFF). The particle size accuracy has been improved significantly in this work by a second-order polynomial calibration. Very good agreement between the FFF data and scanning electron microscopic (SEM) results has been achieved. The effects of particle porosity, pore sizes, and particle sizes on the particle size accuracy in electrical sensing zone (ESZ) analyses have been discussed. It has been demonstrated by computer simulation and experimental measurements that false peaks can be generated in certain particle size regions when the static light scattering (SLS) technique is applied to tightly distributed spherical chromatographic support particles.