کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205811 1493727 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an integrated chromatographic system for on-line digestion and characterization of phosphorylated proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of an integrated chromatographic system for on-line digestion and characterization of phosphorylated proteins
چکیده انگلیسی

The development of an integrated chromatographic system for complete phosphoprotein analysis is described. The digestion of phosphoproteins with trypsin- or pronase-based monolithic bioreactors is carried out on-line with selective enrichment on a TiO2 trap and separation of the produced phosphopeptides by reversed-phase liquid chromatography-multiple mass spectrometry (RPLC/MSn). A detailed study on the selective extraction of peptides with different degrees of phosphorylation on TiO2 cartridges is discussed. This analytical strategy has been optimized using β-casein as a standard phosphoprotein, and then applied to the identification of phosphorylation sites in insulin-like grow factor-binding protein 1 (IGFBP-1) isolated from amniotic fluid.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1183, Issues 1–2, 7 March 2008, Pages 65–75
نویسندگان
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