کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1205946 | 965213 | 2008 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Analysis of basic compounds by reversed-phase liquid chromatography–electrospray mass spectrometry in high-pH mobile phases Analysis of basic compounds by reversed-phase liquid chromatography–electrospray mass spectrometry in high-pH mobile phases](/preview/png/1205946.png)
The separation of basic compounds in high-pH mobile phases results in extended retention, excellent peak shapes and good chromatographic efficiency. A severe decrease in sensitivity with electrospray mass spectrometric detection in positive ion mode (ESI+-MS) is expected under conditions that suppress analyte ionization in solution. We compared the responses of a large number of various basic drugs covering a wide range of hydrophobic (log P 0.09 to 7.6) and basic character (pKa 6.8–10) in LC–ESI+-MS/MS in 0.1% formic acid in water and acetonitrile, to responses in 10 mM ammonium hydrogencarbonate buffers of different pH (7.8–11), and acetonitrile. Contrary to common expectations, high-pH mobile phases do not affect negatively the responses of basic compounds in ESI+. Analyte responses and limits of detection are comparable, or most often better in high pH compared to acidic mobile phases.
Journal: Journal of Chromatography A - Volume 1179, Issue 2, 1 February 2008, Pages 131–144