کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1205949 965213 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rational design of a new one-step purification strategy for Candida antarctica lipase B by ion-exchange chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rational design of a new one-step purification strategy for Candida antarctica lipase B by ion-exchange chromatography
چکیده انگلیسی

A fast and efficient one-step method for purification of lipase B from Candida antarctica by ion-exchange chromatography was developed by rational design. The electrostatic properties of the enzyme were calculated and validated by isoelectric focusing and measurement of the titration curve. C. antarctica lipase B shows an unusual pH profile with a broad isoelectric region from pH 4 to 8. At pH 3 C. antarctica lipase B can be bound to a cation-exchange chromatography column and was purified to homogeneity with a purification factor of 2.4. It was stable at pH 3, the residual activity was still 80% after 6 days incubation at 20 °C. The broad isoelectric region of C. antarctica lipase B is unique as compared to almost all other α/β-hydrolases which have a well-defined isoelectric point. A search in the lipase engineering database resulted in only one further α/β-hydrolase, the Fusarium solani cutinase, which also has a broad isoelectric region.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1179, Issue 2, 1 February 2008, Pages 161–167
نویسندگان
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