کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206153 965221 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of agarose and dextran-grafted agarose strong ion exchangers for the separation of protein aggregates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Comparison of agarose and dextran-grafted agarose strong ion exchangers for the separation of protein aggregates
چکیده انگلیسی

The control of aggregate levels in recombinant protein based drugs is a primary concern during process development and manufacture. In recent years, a novel class of dextran-grafted ion exchange matrices has gained popularity for process scale protein purification due to increased mass transfer rates and higher dynamic binding capacity compared to conventional matrices. Using bovine serum albumin and a monoclonal antibody as model proteins, we studied Sepharose FF and Sepharose XL ion exchangers for the separation of protein aggregates. Experimental results comparing linear gradient elution, stepwise elution, and flow-through chromatography for aggregate separation are described. Differences in performance for the various ion exchangers are discussed and modeled. Strategies for the optimization of protein aggregate separation are provided.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 27, 3 July 2009, Pages 5256–5264
نویسندگان
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