کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206239 | 1493709 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins Lectin affinity chromatography as a tool to differentiate endogenous and recombinant erythropoietins](/preview/png/1206239.png)
چکیده انگلیسی
This work exploits the combination of the lectin affinity chromatography (LAC) with an ultra-sensitive immunochromatographic assay to differentiate several types of erythropoietin (EPO). The chromatographic behaviours of different commercial types of recombinant human EPO (rhEPO), EPO analogues (Aranesp) and urine human EPO (uhEPO) from healthy individuals on eight lectin-Sepharose columns, have been worked out. Results show that when using wheat germ agglutinin (WGA)-Sepharose columns, a careful desorption regime starting with very low concentration (2 mM) of the competitive sugar N-acetylglucosamine (GlcNAc) makes it possible to efficiently distinguish endogenous EPO from recombinant EPO and EPO analogues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1212, Issues 1–2, 28 November 2008, Pages 82–88
Journal: Journal of Chromatography A - Volume 1212, Issues 1–2, 28 November 2008, Pages 82–88
نویسندگان
L. Franco Fraguas, J. Carlsson, M. Lönnberg,