کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206257 | 1493682 | 2012 | 10 صفحه PDF | دانلود رایگان |
Clomiphene, a selective estrogen receptor modulator, is prohibited by World Anti Doping Agency (WADA) out-of-competition and in-competition. As it is extensively metabolized, further investigation of clomiphene metabolic profile will be essential to routine anti-doping analysis. The metabolic pathway and the different metabolites of clomiphene in human urine collected from three healthy volunteers during 1 week were studied by liquid chromatography–quadrupole time-of-flight mass spectrometry (LC–QTOFMS) based on accurate mass measurement. Seven unreported metabolites were identified and characterized, and all of the newly found urinary metabolites belonged to a new metabolic pathway (hydrogenation). An approach for the metabolism study of clomiphene and its analogs by LC–QTOFMS was presented. Two metabolites, 3,4-dihydroxy-dihydro-clomiphene (m/z 440.1991) and 3,4-dihydroxy-dihydro-deethyl-clomiphne (m/z 412.1674), are the potential biomarkers for monitoring oral administration of clomiphene in doping control.
► The metabolic pathway and metabolites of clomiphene in human urine were studied.
► Seven unreported metabolites were identified and characterized by LC–QTOFMS.
► Two newly found metabolites are potential biomarkers in doping control analysis.
Journal: Journal of Chromatography A - Volume 1243, 22 June 2012, Pages 23–32