کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1206322 1493734 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multivariate comparison between peptide mass fingerprints obtained by liquid chromatography–electrospray ionization-mass spectrometry with different trypsin digestion procedures
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Multivariate comparison between peptide mass fingerprints obtained by liquid chromatography–electrospray ionization-mass spectrometry with different trypsin digestion procedures
چکیده انگلیسی

Peptide mass fingerprints were obtained for three different proteins using three different digestion procedures in triplicates with liquid chromatography coupled to electrospray ionization mass spectrometry. For each protein the results were compared with multivariate data analysis (cluster analysis, kernel principal component analysis) and pair-wise contrast evaluation. Clear systematic differences between the digestion procedures were established for all the proteins. The visual presentation of the pair-wise differences between procedures could to some extent be related to the protein fragments, although the main objective was to identify m/z and retention regions in the original peptide maps that should be subject to further exploration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1171, Issues 1–2, 9 November 2007, Pages 69–79
نویسندگان
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