کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1206439 | 1493712 | 2008 | 5 صفحه PDF | دانلود رایگان |
A new method involving matrix solid-phase dispersion (MSPD) extraction and hydrophilic interaction ultra-high-pressure liquid chromatography (HILIC-UHPLC) with photodiode array detection was developed for the determination of carbadox and olaquindox in feed. Separation of carbadox and olaquindox was achieved within 1 min on the 1.7 μm Acquity UPLC BEH HILIC column by using isocratic elution with a mobile phase consisting of 10 mmol L−1 ammonium acetate in acetonitrile–water (95:5, v/v) at a flow rate of 0.5 mL min−1. Optimization of MSPD extraction parameters, such as type of solid sorbent and elution solvent were carried out. Optimal conditions selected for MSPD extraction were: 0.25 g of feed sample, 0.5 g of octadecylsilica as solid sorbent and 10 mL of acetonitrile–methanol (8:2, v/v) as eluting solvent. Both analytes provided average recoveries from spiked feed samples ranging from 89.1 to 98.4% with relative standard deviations less than 10%. Obtained performance characteristics are comparable to those achieved by liquid–liquid extraction-HPLC with the advantages of being simpler and significantly faster.
Journal: Journal of Chromatography A - Volume 1209, Issues 1–2, 31 October 2008, Pages 83–87