کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206545 | 965238 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bilayer microfluidic chip for diffusion-controlled activation of yeast species
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Bilayer microfluidic chip for diffusion-controlled activation of yeast species Bilayer microfluidic chip for diffusion-controlled activation of yeast species](/preview/png/1206545.png)
چکیده انگلیسی
A bilayer microfluidic chip is used, in which multiple laminar streams are generated to define local microenvironments. The bilayer architecture of the microchip separates cell handling and positioning from cell activation by soluble chemicals. Cell activation is diffusion controlled through a porous membrane. By employing time-lapse fluorescence microscopy, gene expression of the enhanced green fluorescent protein (eGFP) in Saccharomyces cerevisiae is studied under various conditions. We demonstrate that the yeast cells remain viable in the microchip for at least 17 h, and that gene expression can be initiated by the supply of the inducer galactose at a spatial precision of a few micrometers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1206, Issue 1, 3 October 2008, Pages 77–82
Journal: Journal of Chromatography A - Volume 1206, Issue 1, 3 October 2008, Pages 77–82
نویسندگان
F. Kurth, C.A. Schumann, L.M. Blank, A. Schmid, A. Manz, P.S. Dittrich,