کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206904 | 965249 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Adsorption behaviour of a quinidine carbamate-based chiral stationary phase: Role of the additive Adsorption behaviour of a quinidine carbamate-based chiral stationary phase: Role of the additive](/preview/png/1206904.png)
In this study, we incorporate the additive properties into the theoretical model of a general preparative chromatographic system; this is normally not done and this limits a proper process optimization. As a model phase system, we used the adsorption of 9H-fluoren-9-ylmethoxycarbonyl-allylglycine (Fmoc-allylglycine) enantiomers on a quinidine carbamate-based chiral stationary phase (anion exchanger) together with a methanol–glacial acetic acid–ammonium acetate eluent. The inverse method was used to measure the competitive adsorption isotherms of both the Fmoc-allylglycine enantiomers as well as the non-detectable additive acetic acid. It was concluded that this enantioselective preparative system is well described by a non-heterogeneous adsorption model and that the loading capacity is very high. The proposed model is valid over a wide range of additive concentrations, which is important for process optimization.
Journal: Journal of Chromatography A - Volume 1216, Issue 16, 17 April 2009, Pages 3480–3487