کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1206992 | 965252 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate–protein complex
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10−5 cm2 V−1 s−1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate–protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1192, Issue 2, 30 May 2008, Pages 289–293
Journal: Journal of Chromatography A - Volume 1192, Issue 2, 30 May 2008, Pages 289–293
نویسندگان
Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba,