کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207099 | 965255 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process](/preview/png/1207099.png)
چکیده انگلیسی
An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 13, 27 March 2009, Pages 2683–2688
Journal: Journal of Chromatography A - Volume 1216, Issue 13, 27 March 2009, Pages 2683–2688
نویسندگان
Jeremy Lee, Anne Tscheliessnig, Allen Chen, Yih Yean Lee, Gloria Adduci, Andre Choo, Alois Jungbauer,