کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207133 965257 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High speed gradient elution reversed phase liquid chromatography of bases in buffered eluents: Part II. Full equilibrium
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High speed gradient elution reversed phase liquid chromatography of bases in buffered eluents: Part II. Full equilibrium
چکیده انگلیسی

In this work we determined when the state of thermodynamic (full) equilibrium, i.e. time-invariate solute retention, was achieved in gradient elution reversed-phase chromatography. We investigated the effects of flow rate, temperature, organic modifier, buffer type/concentration, stationary phase type, n-butanol as eluent additive, and pore size. We also measured how selectivity varied with reequilibration time. Stationary phase wetting and the ability of the stationary phase to resist changes in pH strongly affect the time needed to reach full equilibrium. For example, full equilibrium is realized with many endcapped stationary phases after flushing with only two column volumes of acetonitrile–water containing 1% (v/v) n-butanol and 0.1% (v/v) trifluoroacetic acid. Trends in retention time (<0.010 min) and selectivity become quite small after only five column volumes of reequilibration. We give practical guidelines that provide fast full equilibrium for basic compounds when chromatographed in buffered eluents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1192, Issue 1, 23 May 2008, Pages 54–61
نویسندگان
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