کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207231 | 1493713 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of an improved proteomics method, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
To identify age-related proteins in small regions of mouse brain, we improved a proteomics approach, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry (FD–LC–MS/MS), and applied the method to the differential proteome analysis of aging in cerebral cortex, hippocampus and brainstem. The method showed good accuracy with RSDs < 10% for between-day protein peak heights, and much better sensitivity for the detection of proteins compared to other proteomics approaches. The existence of 28 regionally specific age-related proteins in mouse brain was demonstrated. These results verified that the small brain regions could be the targets for proteome analysis by the FD–LC–MS/MS method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1208, Issues 1–2, 24 October 2008, Pages 147–155
Journal: Journal of Chromatography A - Volume 1208, Issues 1–2, 24 October 2008, Pages 147–155
نویسندگان
Hiromichi Asamoto, Tomoko Ichibangase, Kazuo Uchikura, Kazuhiro Imai,