کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207231 | 1493713 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of an improved proteomics method, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of an improved proteomics method, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain Application of an improved proteomics method, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain](/preview/png/1207231.png)
چکیده انگلیسی
To identify age-related proteins in small regions of mouse brain, we improved a proteomics approach, fluorogenic derivatization–liquid chromatography–tandem mass spectrometry (FD–LC–MS/MS), and applied the method to the differential proteome analysis of aging in cerebral cortex, hippocampus and brainstem. The method showed good accuracy with RSDs < 10% for between-day protein peak heights, and much better sensitivity for the detection of proteins compared to other proteomics approaches. The existence of 28 regionally specific age-related proteins in mouse brain was demonstrated. These results verified that the small brain regions could be the targets for proteome analysis by the FD–LC–MS/MS method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1208, Issues 1–2, 24 October 2008, Pages 147–155
Journal: Journal of Chromatography A - Volume 1208, Issues 1–2, 24 October 2008, Pages 147–155
نویسندگان
Hiromichi Asamoto, Tomoko Ichibangase, Kazuo Uchikura, Kazuhiro Imai,