کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1207345 | 965263 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of a preparative-scale, recirculating isoelectric trapping device for the isolation and enrichment of acidic proteins in bovine serum
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A recirculating, preparative-scale isoelectric trapping device, developed for the binary isoelectric trapping separation of proteins has been used to desalt, isolate and enrich the pIÂ <Â 4 protein fraction from a 150Â mL sample of bovine serum. Subsequent re-separation of the 2Â <Â pIÂ <Â 4 fraction with pH 3.0, 3.5 and 3.9 buffering membranes resulted in distinct, narrow pI fractions whose components could be readily analyzed by reversed-phase HPLC, even though they were below the detection limit in the original bovine serum sample. The entire isoelectric trapping process (from desalting to collection of the final, narrow pI fractions) took only 7Â h, indicating the potential of the recirculating, preparative-scale isoelectric trapping device as a front-end component in the proteomic work-flow when sufficiently large samples are available.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1155, Issue 2, 6 July 2007, Pages 237-241
Journal: Journal of Chromatography A - Volume 1155, Issue 2, 6 July 2007, Pages 237-241
نویسندگان
Evan Shave, Gyula Vigh,