کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1207911 1493723 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultra-performance liquid chromatographic analysis of amino acids in protein hydrolysates using an automated pre-column derivatisation method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ultra-performance liquid chromatographic analysis of amino acids in protein hydrolysates using an automated pre-column derivatisation method
چکیده انگلیسی

In the present study, the changeover from the Pico·Tag HPLC method to the AccQ·Tagultra UPLC method for the analysis of amino acids in casein and bovine serum albumine hydrolysates is described. The total chromatographic run time of the AccQ·Tagultra UPLC method was only 40% of the time required for the Pico·Tag HPLC method. Quantitative results of both methods for casein and bovine serum albumine hydrolysates compared fairly well. The derivatisation protocol for the formation of AQC derivatives of amino acids was automated using a Gilson Model 215 liquid handler. Comparison of the manual derivatisation protocol with the automated protocol showed lower coefficients of variation for the latter. Combination of the AccQ·Tagultra UPLC method and automated derivatisation resulted in improved throughput compared to the Pico·Tag HPLC method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1189, Issues 1–2, 2 May 2008, Pages 406–409
نویسندگان
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