کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208006 965287 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of plant viral and satellite double-stranded RNAs on DEAE monoliths
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification of plant viral and satellite double-stranded RNAs on DEAE monoliths
چکیده انگلیسی

Replicative double-stranded RNA (dsRNA) is useful in preliminary identification of Cucumber mosaic virus and its satellite RNA (satRNA). This plant pathogen complex yields sufficient quantity of the replicative RNA form that can be isolated by chromatography on chemically unmodified graded cellulose powder (CF-11). In this work, much faster and more efficient procedure using DEAE monoliths was developed in which dsRNA was separated from other species in total nucleic acids extract originating from the infected plant tissue. The developed chromatographic method revealed the pathogens’ presence in only 15 min, avoiding nucleic acid precipitation and electrophoretic analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1144, Issue 1, 9 March 2007, Pages 111–119
نویسندگان
, , , , ,