کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208046 965288 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enantiomeric resolution of bupivacaine by high-performance liquid chromatography and chiroptical detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enantiomeric resolution of bupivacaine by high-performance liquid chromatography and chiroptical detection
چکیده انگلیسی
This work reports a new analytical procedure for the separation and determination of the enantiomers of bupivacaine and the determination of the enantiomeric purity. The isomers were separated using a Chirex 3020 (250 mm × 4.6 mm) with a mobile phase of n-hexane:dichloroethane:ethanol (82:9:9, v/v/v) at a flow-rate of 1 ml min−1 and UV, polarimetric and circular dichroism (CD) detection. Obtained retention times were 5.93 and 7.53 min (R and S) with a resolution of Rs = 2.36. Precisions (RSD) were 1.83 and 2.02% (CD detection) and 3.07 and 1.26% (UV detection) for R- and S-enantiomers, respectively (at 10 μg level). Detection limits were 0.5 and 0.5 μg (R and S) with CD detection, and 0.9 and 0.3 μg with UV detection. Polarimetric detection was inadequate to perform a quantitative method at similar concentration ranges as UV and CD because of poor sensitivity. A procedure for determination of enantiomeric purity using a conventional chromatographic column (RP18, Luna) coupled to a CD detector and anisotropy factor (CD/UV) as analytical signal was also developed. Obtained results show that RSDs of 6.7-1.6% were obtained in the range of 0-100% enantiomeric purity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1188, Issue 2, 25 April 2008, Pages 314-317
نویسندگان
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