کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208088 965290 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera
چکیده انگلیسی

This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a charge-coupled device camera. Limits of detection were found to be 5 ng on a wetted plate and 3 ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r2 values > 0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient (ɛapp) of 10 AU m2 g−1 at 254 nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1182, Issue 2, 29 February 2008, Pages 219–225
نویسندگان
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