The influence of salt type on the retention of bovine serum albumin in ion-exchange chromatography

In this paper, an analysis of the influence of the salt types, NaCl, NaCH3COO, Na2SO4 and Na3C6H5O7, on the isocratic retention behaviour of bovine serum albumin (BSA) on two anion-exchangers media (Source 30Q and TSK Gel Super Q 5 PW) has been presented. The retention data demonstrated that the mechanism of protein retention in ion-exchange chromatography (IEC) involves interactions between the protein solute, the mobile phase constituents and the stationary phase. The effect of protein activity coefficient in the mobile phase on the protein retention volumes is verified.