کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1208351 | 965302 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of 3R,3â²R-zeaxanthin in plant derived food by a diastereomeric dilution assay applying chiral high-performance liquid chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
For quantification of 3R,3â²R-zeaxanthin in plant derived food, a new diastereomeric dilution assay (DIDA) was developed using chiral high-performance liquid chromatography coupled to diode-array detection (HPLC/DAD). As diastereomeric internal standard (I.S.), 3R,3â²S-(meso)-zeaxanthin was applied. DIDA showed sufficient sensitivity with low limits of detection and quantification (18 μg 100 gâ1 and 45 μg 100 gâ1), a good inter-assay coefficient of variation (2.4%), determined by analysis of a certified BCR reference material, and a good recovery (94.7%). To prove the applicability of DIDA, various plant materials were anaylzed, showing that DIDA is highly advisable if saponification is applied during work-up to hydrolyze xanthophyll esters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1137, Issue 2, 29 December 2006, Pages 216-222
Journal: Journal of Chromatography A - Volume 1137, Issue 2, 29 December 2006, Pages 216-222
نویسندگان
Jörg Schlatterer, Svenja Maurer, Dietmar E. Breithaupt,