کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208360 1493729 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Low-voltage electromembrane extraction of basic drugs from biological samples
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Low-voltage electromembrane extraction of basic drugs from biological samples
چکیده انگلیسی

The present work has for the first time demonstrated electromembrane extraction (EME) at voltages obtainable by common batteries. Five basic drugs were extracted from acidified aqueous sample solutions, across a supported liquid membrane (SLM) consisting of 1-isopropyl-4-nitrobenzene impregnated in the walls of a hollow fiber, and into an acidified aqueous acceptor solution present inside the lumen of the hollow fiber with potential differences of 1–10 V applied over the SLM. Extractions from 1 ml standard solutions prepared in 10 mM HCl for 5 min and with a potential of 10 V demonstrated analyte recoveries of 50–93% in 25 μl of 10 mM HCl as acceptor solution. This corresponds to enrichment factors of 20–37. Similar results were obtained with a common 9 V battery as power supply. Recoveries from low-voltage EME on human plasma, urine, and breast milk diluted with acetate buffer (pH 4) demonstrated recoveries in the range of 37–55% after 5 min of extraction. Excellent selectivity was demonstrated as no interfering peaks were detected. Standard curves in the range of 0.0625–0.625 μg/ml demonstrated correlation coefficients of 0.994–0.999. Extraction recoveries from human plasma, urine or breast milk were not found to be sensitive towards individual variations. The results show that low-voltage EME has a future potential as a simple, selective, and time-efficient sample preparation technique of biological fluids.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1180, Issues 1–2, 8 February 2008, Pages 1–9
نویسندگان
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