کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1208634 965311 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of an Fc-fusion biologic: Clearance of multiple product related impurities by hydrophobic interaction chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification of an Fc-fusion biologic: Clearance of multiple product related impurities by hydrophobic interaction chromatography
چکیده انگلیسی

An hydrophobic interaction chromatography step was developed for the large-scale production of an Fc-fusion biologic. Two abundant product-related impurities were separated from the active monomer using a Butyl resin and a simple step-wash and step-elution strategy. Capacity and resolution of the HIC step was optimal when sodium sulfate was employed as the lyotropic salt and pore size of the Butyl resin was 750 Å. Factorial analysis identified critical parameters for the Butyl chromatography and an operating window capable of delivering high product quality and yield over a broad column loading range.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1177, Issue 2, 11 January 2008, Pages 265–271
نویسندگان
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