کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1209149 965330 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sample capacity in preparative high-speed counter-current chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sample capacity in preparative high-speed counter-current chromatography
چکیده انگلیسی

High-speed counter-current chromatography (HSCCC) is a versatile technique in preparative separation and purification of pure compounds from complex matrices. As a preparative chromatography, there is a need to maximize the column production. Based on the plate theory of Van Deemter, the effect of the sample load on the separation was investigated in a preparative HSCCC with a 1000 ml column capacity. The test samples of hydroquinone, pyrocatechol and phenol were separated using a two-phase solvent system of n-hexane–ethyl acetate–ethanol–water (1:1:1:1, v/v/v/v) at different sample loads. The results showed that for the case of HSCCC, the agreement of the effect of sample load on peak height and peak width between the Van Deemter's theory and the experiments is excellent. Furthermore, the factors limiting the mass load, including the resolution between the peaks, the partition isotherm and the solute solubility were also discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1146, Issue 2, 6 April 2007, Pages 186–192
نویسندگان
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