کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209472 | 1493757 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: New Q membrane scale-down model for process-scale antibody purification New Q membrane scale-down model for process-scale antibody purification](/preview/png/1209472.png)
Process-scale antibody production requires polishing steps with extremely high product throughput and robust operation. In this communication, the Sartobind Q membrane adsorber for process-scale antibody production is evaluated as an alternative to Q column chromatography. Although the capacity seen with large-scale membrane adsorbers is competitive with column chromatography, the same throughput is not achieved with the current scale-down models. The operational issues currently found in membrane scale-down models, including backpressure, which significantly compromises the membrane's capacity, were examined. A new scale-down model was designed to mimic the liquid flow path found in the large-scale capsule, and a new process capacity equivalent at both small and large scale was successfully achieved. Results of a 4-model virus study with a redesigned Sartobind Q absorber scale-down model at the new process capacity are presented.
Journal: Journal of Chromatography A - Volume 1134, Issues 1–2, 17 November 2006, Pages 66–73