کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209485 | 1493757 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection](/preview/png/1209485.png)
چکیده انگلیسی
A fully automated, qualitative screening HPLC method for the identification of basic compounds in urine has been developed. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange (SCX) columns (2 Ã LunaSCX, 150 mm Ã 4.6 mm, 5 μm) under isocratic conditions. The mobile phase consisted of a mixture of potassium dihydrogenphosphate buffer (pH 2.3) and acetonitrile. The use of photodiode-array detection (DAD, λ = 190-800 nm) gave access to a library of approximately 2600 toxicologically relevant compounds. The validated method is reliable, simple and in addition successfully proven with the analysis of real biological specimen for the routine use.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1134, Issues 1â2, 17 November 2006, Pages 177-185
Journal: Journal of Chromatography A - Volume 1134, Issues 1â2, 17 November 2006, Pages 177-185
نویسندگان
Lena Schönberg, Thomas Grobosch, Dagmar Lampe, Charlotte Kloft,