کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1209580 1493759 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a reversed-phase high-performance liquid chromatography method for the analysis of components from a closed-loop insulin delivery system
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of a reversed-phase high-performance liquid chromatography method for the analysis of components from a closed-loop insulin delivery system
چکیده انگلیسی

A reversed-phase HPLC method has been developed which enables separation of the three components of a closed-loop insulin delivery system, namely concanavalin A methacrylamide (Con A-MA), dextran methacrylate (Dex-MA) and bovine insulin. The analysis of Con A-MA represents a significant challenge due to the formation of multiple conformations on contact with the chromatographic surface and the mobile phase. The extent of conformational change is shown to be dependent on a number of parameters: column temperature, mobile phase pH, contact time with the chromatographic surface, salt type and concentration and the organic modifier. By manipulation of these variables, protein denaturation can be minimised and recovery improved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1132, Issues 1–2, 3 November 2006, Pages 117–123
نویسندگان
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