کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1209778 | 1493761 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Separation method based on affinity reaction between magnetic and nonmagnetic particles for the analysis of particles and biomolecules Separation method based on affinity reaction between magnetic and nonmagnetic particles for the analysis of particles and biomolecules](/preview/png/1209778.png)
A separation method is reported for particle and biochemical analysis based on affinity interactions between particle surfaces under magnetic field. In this method, magnetic particles with immunoglobulin G (IgG) or streptavidin on the surface are flowed through a separation channel to form a deposition matrix for selectively capturing nonmagnetic analytes with protein A or biotin on the surface due to specific antigen (Ag)–antibody (Ab) interactions. This separation method was demonstrated using model reactions of IgG–protein A and streptavidin–biotin on particle surface. The features of this new separation method are (1) the deposited Ag–Ab complex can be examined and further analyzed under the microscope, (2) a kinetic study of complex binding is possible, and (3) the predeposited matrix can be formed selectively and changed easily. The detection limits were about 10−11 g. The running time was less than 10 min. The selectivities of studied particles were 94% higher than those of label-controlled particles. This method extends the applications of analytical magnetapheresis to nonmagnetic particles. Preliminary study shows that this separation method has a great potential to provide a simple, fast, and selective analysis for particles, blood cells, and immunoassay related applications.
Journal: Journal of Chromatography A - Volume 1130, Issue 2, 20 October 2006, Pages 227–231