کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1209867 1493764 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
How does column packing microstructure affect column efficiency in liquid chromatography?
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
How does column packing microstructure affect column efficiency in liquid chromatography?
چکیده انگلیسی

Full three-dimensional computer simulations of the fluid flow and dispersion characteristics of model nonporous chromatographic packings are reported. Interstitial porosity and packing defects are varied in an attempt to understand the chromatographic consequences of the packing microstructure. The tracer zone dispersion is calculated in the form of plate height as a function of fluid velocity for seven model particle packs where particles are selectively removed from the packs in clusters of varying size and topology. In an attempt to examine the consequences of loose but random packs, the velocities and zone dispersion of seven defect-free packs are simulated over the range 0.36≤ɛ≤0.500.36≤ɛ≤0.50, where ɛɛ is the interstitial porosity. The results indicate that defect-free loose packings can give good chromatographic efficiency but the efficiency can vary depending on subtle details of the pack. When the defect population increases, the zone dispersion increases accordingly. For a particle pack where 6% of the particles are removed from an ɛ=0.36ɛ=0.36 pack, ≈≈ 33% of the column efficiency is lost. These results show that it is far more important in column packing to prevent defect sites leading to inhomogeneous packing rather than obtaining the highest density pack with the smallest interstitial void volume.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1126, Issues 1–2, 8 September 2006, Pages 58–69
نویسندگان
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