کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1210083 | 1493775 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Separation of single-stranded DNAs using DNA conjugates having different migration properties in capillary electrophoresis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A probe-regulated simultaneous separation (PRESS) using capillary electrophoresis (CE) was developed for separating single-stranded (ss) DNAs. We synthesized two DNA conjugate probes, -(5â²-TGTGTGTGT-3â²)p-AAmq- and -(5â²-GCCACCAGC-3â²)m-AAmn-, by copolymerizing 5â²-methacryloyl-modified ssDNA with acrylamide (AAm), and characterized them in detail. The two probes showed lower electrophoretic mobilities than 5â²-methacryloyl-modified ssDNAs. Furthermore, -(5â²-TGTGTGTGT-3â²)p-AAmq- showed slightly faster electrophoretic mobility toward the anode than -(5â²-GCCACCAGC-3â²)m-AAmn- due to its higher molar fraction of negatively-charged ssDNA. We successfully separated target ssDNAs having the same chain length by using two ssDNA conjugate probes that showed different electrophoretic mobilities, although the separation of these ssDNAs was difficult in conventional capillary electrophoresis systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1106, Issues 1â2, 17 February 2006, Pages 118-123
Journal: Journal of Chromatography A - Volume 1106, Issues 1â2, 17 February 2006, Pages 118-123
نویسندگان
Y. Murakami, M. Maeda,