کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210083 1493775 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation of single-stranded DNAs using DNA conjugates having different migration properties in capillary electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Separation of single-stranded DNAs using DNA conjugates having different migration properties in capillary electrophoresis
چکیده انگلیسی
A probe-regulated simultaneous separation (PRESS) using capillary electrophoresis (CE) was developed for separating single-stranded (ss) DNAs. We synthesized two DNA conjugate probes, -(5′-TGTGTGTGT-3′)p-AAmq- and -(5′-GCCACCAGC-3′)m-AAmn-, by copolymerizing 5′-methacryloyl-modified ssDNA with acrylamide (AAm), and characterized them in detail. The two probes showed lower electrophoretic mobilities than 5′-methacryloyl-modified ssDNAs. Furthermore, -(5′-TGTGTGTGT-3′)p-AAmq- showed slightly faster electrophoretic mobility toward the anode than -(5′-GCCACCAGC-3′)m-AAmn- due to its higher molar fraction of negatively-charged ssDNA. We successfully separated target ssDNAs having the same chain length by using two ssDNA conjugate probes that showed different electrophoretic mobilities, although the separation of these ssDNAs was difficult in conventional capillary electrophoresis systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1106, Issues 1–2, 17 February 2006, Pages 118-123
نویسندگان
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