کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1210162 | 1493768 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cationic lipid vesicles as coating precursors in capillary electrochromatography: Separation of basic proteins and neutral steroids
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cationic lipid vesicles as coating precursors in capillary electrochromatography: Separation of basic proteins and neutral steroids Cationic lipid vesicles as coating precursors in capillary electrochromatography: Separation of basic proteins and neutral steroids](/preview/png/1210162.png)
چکیده انگلیسی
1,2-Dioleyl-3-trymethylammoniumpropane (DOTAP) lipid vesicles were employed as coating precursors to obtain a semipermanent cationic lipid bilayer in silica capillary. The coating procedure was relatively fast and simple. Reliable results for the separation of four basic proteins (α-chymotrypsinogen A, ribonuclease A, cytochrome C, lysozyme) were obtained by using an acetate buffer under acidic conditions. The RSDs of the migration times were not higher than 0.5% run-to-run and about 1% day-to-day (3 days), while the RSDs of the peak areas were within 7% day-to-day (3 days). The day-to-day RSD of the EOF mobility of about 1%, confirmed that the DOTAP coating was stable for the separation of basic proteins, under acidic buffers. In addition to basic proteins the DOTAP coating was found suitable under acidic conditions for the repeatable separation of neutral steroids. The potential of DOTAP as a carrier in background electrolyte solution was studied.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1119, Issues 1â2, 30 June 2006, Pages 163-169
Journal: Journal of Chromatography A - Volume 1119, Issues 1â2, 30 June 2006, Pages 163-169
نویسندگان
Matteo Bonoli, Sami J.O. Varjo, Susanne K. Wiedmer, Marja-Liisa Riekkola,