کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210552 965377 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative evaluation of thin-layer chromatography with image background estimation based on charge-coupled device imaging
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitative evaluation of thin-layer chromatography with image background estimation based on charge-coupled device imaging
چکیده انگلیسی

The main limitation of image processing based on charge-coupled device (CCD) imaging in quantitative evaluation of thin-layer chromatography (TLC) is its nonuniform illumination. This paper presents a novel method to solve the problem. First, the background is estimated by employing the cubic spline surface construction. Then the estimated background is subtracted from the original image to make the image segmentation; Finally, the gray level integration for each object is calculated, which is proportional to the value of samples. Experiment shows that the linear correlation coefficient between gray integration and concentration of samples is 0.9978.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1109, Issue 2, 24 March 2006, Pages 273–278
نویسندگان
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