کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1210552 | 965377 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative evaluation of thin-layer chromatography with image background estimation based on charge-coupled device imaging
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The main limitation of image processing based on charge-coupled device (CCD) imaging in quantitative evaluation of thin-layer chromatography (TLC) is its nonuniform illumination. This paper presents a novel method to solve the problem. First, the background is estimated by employing the cubic spline surface construction. Then the estimated background is subtracted from the original image to make the image segmentation; Finally, the gray level integration for each object is calculated, which is proportional to the value of samples. Experiment shows that the linear correlation coefficient between gray integration and concentration of samples is 0.9978.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1109, Issue 2, 24 March 2006, Pages 273–278
Journal: Journal of Chromatography A - Volume 1109, Issue 2, 24 March 2006, Pages 273–278
نویسندگان
Li Zhang, Xinggang Lin,