کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1210624 | 965379 | 2009 | 5 صفحه PDF | دانلود رایگان |

Size-exclusion chromatography with on-line synchrotron radiation solution small-angle X-ray scattering optics, absorbance and/or refractive index detectors was evaluated by protein characterizations. The radius of gyration value and zero-angle scattering intensity of protein molecules eluted from the chromatography column were estimated using this measurement system. In addition, the characterization of the conformation of the eluted proteins was demonstrated for hen egg lysozyme and bovine submaxillary mucin. The present technique will be useful for not only the determination of the radius of gyration value and molecular weight of proteins with dimensions of 1–10 nm, but also for the structural characterization of the macromolecules during the chromatography.
Journal: Journal of Chromatography A - Volume 1216, Issue 44, 30 October 2009, Pages 7461–7465