کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210624 965379 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Size-exclusion chromatography combined with small-angle X-ray scattering optics
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Size-exclusion chromatography combined with small-angle X-ray scattering optics
چکیده انگلیسی

Size-exclusion chromatography with on-line synchrotron radiation solution small-angle X-ray scattering optics, absorbance and/or refractive index detectors was evaluated by protein characterizations. The radius of gyration value and zero-angle scattering intensity of protein molecules eluted from the chromatography column were estimated using this measurement system. In addition, the characterization of the conformation of the eluted proteins was demonstrated for hen egg lysozyme and bovine submaxillary mucin. The present technique will be useful for not only the determination of the radius of gyration value and molecular weight of proteins with dimensions of 1–10 nm, but also for the structural characterization of the macromolecules during the chromatography.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1216, Issue 44, 30 October 2009, Pages 7461–7465
نویسندگان
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