کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210812 965414 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of a liquid chromatography–tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Validation of a liquid chromatography–tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum
چکیده انگلیسی

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC–MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC–MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1210, Issue 1, 7 November 2008, Pages 55–59
نویسندگان
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