کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1210906 965434 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection
چکیده انگلیسی

A whole-column detection (WCD) system was fabricated using the parts from a typical A4 size optical scanner. Optimum layout of the fluorescence tube, beam condenser and photon transducers from the scanner made an effective devise to examine the dynamic chromatographic separation process. This system was applied in monitoring peak crossover in an ion-pair chromatographic elution. A stage-wise gradient was conducted in eluting a mixture of New Coccine and Carmoisine. Compound New Coccine lagging behind after sample injection moved passing Carmoisine at the column outlet. The complete on-column elution process could be observed directly using this detection system. This inexpensive and rugged WCD system provided a 0.3 mm spatial resolution and a 3.6 ms temporal resolution and was proven to be an adequate device to directly monitor solute retention behavior in the liquid chromatography column.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1201, Issue 1, 1 August 2008, Pages 128–131
نویسندگان
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