کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211028 965458 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of the ubiquinol-10 and ubiquinone-10 (coenzyme Q10) in human serum by liquid chromatography tandem mass spectrometry to evaluate the oxidative stress
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of the ubiquinol-10 and ubiquinone-10 (coenzyme Q10) in human serum by liquid chromatography tandem mass spectrometry to evaluate the oxidative stress
چکیده انگلیسی

A method for the rapid and simultaneous determination of ubiquinone-10 (coenzyme Q10, CoQ10) and the reduced form ubiquinol-10 (CoQ10H2) in human serum by LC–MS–MS with electrospray ionization (ESI) in the positive mode is here proposed. High selective identification and sensitive quantitation of both analytes have been carried out by monitoring the transition from the corresponding precursor ion to the product ion. Prior to the chromatographic analysis, serum samples (100 μl) were subject to a conventional pre-treatment based on protein precipitation, liquid–liquid extraction, evaporation to dryness and reconstitution with 95:5 methanol/hexane (v/v). The overall method has enabled to achieve low detection limits – 5.49 and 15.8 ng/ml for CoQ10 and CoQ10H2, respectively – which were estimated with serum. The accuracy and potential matrix effects have been studied with spiked serum resulting recoveries between 92.82 and 106.97%. The proposed method has been applied to serum samples from healthy middle-age women, in which the CoQ10H2/CoQ10 ratio has been used as marker of oxidative stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1175, Issue 2, 21 December 2007, Pages 242–248
نویسندگان
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