کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1211561 1493771 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of the influence of interdetector volume on local calibration in size exclusion chromatography with dual multiangle-light-scattering/concentration detection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Analysis of the influence of interdetector volume on local calibration in size exclusion chromatography with dual multiangle-light-scattering/concentration detection
چکیده انگلیسی
A comparison of the s- and w-detections of molecular weight, a necessary condition for precise determination of interdetector volume in size exclusion chromatography of polymers with the dual light scattering-concentration detection, is examined. From the theoretical analysis for a polymer with the log-normal molecular weight distribution it follows that the error in determination of the local calibration, i.e., of a logarithmic dependence of molecular weight on elution volume obtained by the s-detection, from the known dependence of radius of gyration on elution volume and of molecular weight on the radius of gyration, remains linear and is slightly shifted with respect to that obtained by the w-detection (dual detection giving the weight-average molecular weight) towards higher molecular weights, the difference being below the experimental error. It also follows that the error in the slope of local calibration found by the w-detection is given by the ratio of the error in interdetector volume and the variance of the elution curve. This is demonstrated on several polymer samples with symmetric elution curves of polymer samples differing in polydispersity indices. The range of this rule depends on the broadness and symmetry of elution curves. The precision of the interdetector volume determination depends therefore strongly on the M¯w/M¯n ratio of the sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1113, Issues 1–2, 28 April 2006, Pages 162-166
نویسندگان
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