کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1211963 | 1494037 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of atorvastatin and valsartan in human plasma by solid-based disperser liquid-liquid microextraction followed by high-performance liquid chromatography-diode array detection
ترجمه فارسی عنوان
تعیین همزمان اتورواستاتین و والسراتان در پلاسمای انسانی با استفاده از میکرو اکستراکت مایع با جامد و شناسایی آرایه دیود کروماتوگرافی مایع
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کلمات کلیدی
AALLMERSDSPMESDMEDLLMELOQSPELPMEDispersive liquid–liquid microextraction - Microextraction مایعات مایع مایعAtorvastatin - آتورواستاتینSolid phase extraction - استخراج فاز جامدrelative standard deviation - انحراف استاندارد نسبیSolid phase microextraction - بیوفیزیک فاز جامدLiquid phase microextraction - بیوفیزیک فاز مایعdiode array detection - تشخیص آرایه دیودSingle drop microextraction - تک قطره microextractionLOD یا Limit of detection - حد تشخیصlimit of quantification - محدودیت اندازه گیریlimit of detection - محدودیت تشخیصBiological samples - نمونه های بیولوژیکیvalsartan - والسارتانhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کارا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A simple, sensitive, and efficient method has been developed for simultaneous estimation of valsartan and atorvastatin in human plasma by combination of solid-based dispersive liquid-liquid microextraction and high performance liquid chromatography-diode array detection. In the proposed method, 1,2-dibromoethane (extraction solvent) is added on a sugar cube (as a solid disperser) and it is introduced into plasma sample containing the analytes. After manual shaking and centrifugation, the resultant sedimented phase is subjected to back extraction into a small volume of sodium hydrogen carbonate solution using air-assisted liquid-liquid microextraction. Then the cloudy solution is centrifuged and the obtained aqueous phase is transferred into a microtube and analyzed by the separation system. Under the optimal conditions, extraction recoveries are obtained in the range of 81-90%. Calibration curves plotted in drug-free plasma sample are linear in the ranges of 5-5000 μg Lâ1 for valsartan and 10-5000 μg Lâ1 for atorvastatin with the coefficients of determination higher than 0.997. Limits of detection and quantification of the studied analytes in plasma sample are 0.30-2.6 and 1.0-8.2 μg Lâ1, respectively. Intra-day (n = 6) and inter-days (n = 4) precisions of the method are satisfactory with relative standard deviations less than 7.4% (at three levels of 10, 500, and 2000 μg Lâ1, each analyte). These data suggest that the method can be successfully applied to determine trace amounts of valsartan and atorvastatin in human plasma samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1017â1018, 1 April 2016, Pages 62-69
Journal: Journal of Chromatography B - Volumes 1017â1018, 1 April 2016, Pages 62-69
نویسندگان
Mir Ali Farajzadeh, Parisa Khorram, Azar Pazhohan,