Comparison of retention properties of stationary phases imitated cell membrane in RP HPLC

• Stationary phases imitated cell membrane were compared in RP LC.
• log kw parameters on both stationary phases are very well correlated.
• Amino-P-C18 allows prediction of drug interaction with the cell membrane.

Chromatographic properties of two columns was compared: commercial IAM.PC.DD2 that imitates the cell membrane and home-made Amino-P-C18 (N,O-dialkylphosphoramidate C18). The comparison has been done by correlation of retention (log kw parameters) of a series of solutes: hydrophobic (alkyl benzene derivatives and PAHs) and polar, with both acidic (flavonoids) and basic (nucleosides and nucleic bases) character. The slope of correlation plots for hydrophobic compounds and polar basic was very close to 1.0 that confirms the chromatographic similarity. Only for flavonoids the slope of correlation plot was 1.5. For hydrophobic compound retention parameters log kw were also correlated with hydrophobic parameter log P with very good determination coefficients.