کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1212445 1494080 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Urine homogentisic acid and tyrosine: Simultaneous analysis by liquid chromatography tandem mass spectrometry
ترجمه فارسی عنوان
اسید هموگلوبین ادراری و تیروزین: تجزیه و تحلیل همزمان با کروماتوگرافی مایع اسپکترومتری دو طرفه
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Developed LC–MS/MS method for homogentisic acid and tyrosine in human urine.
• Validated in both acidified and non-acidified urine matrices.
• Applicable for diagnosing patients with alkaptonuria.
• Applicable for monitoring response to therapy in treatment of alkaptonuria.
• Validated according to European Medicines Agency Guidelines.

Alkaptonuria (AKU) is a rare debilitating autosomal recessive disorder of tyrosine metabolism. Deficiency of homogentisate 1,2-dioxygenase results in increased homogentisic acid (HGA) which although excreted in gram quantities in the urine, is deposited as an ochronotic pigment in connective tissues, especially cartilage. Ochronosis leads to a severe, early-onset form of osteoarthritis, increased renal and prostatic stone formation and hardening of heart vessels. Treatment with the orphan drug, Nitisinone, an inhibitor of the enzyme 4-hydroxyphenylpyruvate dioxygenase has been shown to reduce urinary excretion of HGA, resulting in accumulation of the upstream pre-cursor, tyrosine. Using reverse phase LC–MS/MS, a method has been developed to simultaneously quantify urinary HGA and tyrosine. Using matrix-matched calibration standards, two product ion transitions were identified for each compound and their appropriate isotopically labelled internal standards. Validation was performed across the AKU and post-treatment concentrations expected. Intrabatch accuracy for acidified urine was 96–109% for tyrosine and 94–107% for HGA; interbatch accuracy (n = 20 across ten assays) was 95–110% for tyrosine and 91–109% for HGA. Precision, both intra- and interbatch was <10% for tyrosine and <5% for HGA. Matrix effects observed with acidified urine (12% decrease, CV 5.6%) were normalised by the internal standard. Tyrosine and HGA were proved stable under various storage conditions and no carryover, was observed. Overall the method developed and validated shows good precision, accuracy and linearity appropriate for the monitoring of patients with AKU, pre and post-nitisinone therapy

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 963, 15 July 2014, Pages 106–112
نویسندگان
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