کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212470 | 1494086 | 2014 | 5 صفحه PDF | دانلود رایگان |
• We established HPLC method for the determination of cinepazide maleate in rat plasma firstly.
• We showed the pharmacokinetic parameter of cinepazide maleate after intravenous administration firstly.
• This HPLC method was reliable, sensitive and easy to implement.
• Cinepazide maleate injection were absorbed and eliminated quickly in rat, and the pharmacokinetic showed dose-dependence profile.
• Injection of cinepazide maleate can improve the bioavailability.
A simple and reliable high performance liquid chromatographic (HPLC) method has been developed and validated to quantify cinepazide maleate, a calcium blocker, in rat plasma. Cinepazide maleate and Tinidazole (internal standard) have been extracted by a simple liquid–liquid extraction before injection into chromatographic system. Chromatographic separation was achieved on a reversed phase C18 column with a mobile phase consisted of a water mixture of 10 mM potassium dihydrogen phosphate (pH = 4.5):methanol (40:60, v/v), pumped at flow rate of 1.0 mL/min, and detected at 303 nm. The method exhibited a linear range of 0.12–120 μg/mL in blank rat plasma, with the lower detection limit of 0.06 μg/mL. The method was statistically validated for linearity, accuracy, precision, selectivity and stability following FDA guidelines. The intra- and inter-assay coefficients of variation did not exceed ±15% from the nominal concentration. The accuracy of cinepazide maleate was within ±15% of the theoretical value. The assay has been applied successfully in a pharmacokinetic study of cinepazide maleate after a single intravenous at three doses in rat. And cinepazide maleate injection can improve the bioavailability of cinepazide maleate greatly, and has a dose-dependence profile in rats.
Journal: Journal of Chromatography B - Volume 957, 15 April 2014, Pages 105–109