|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|1212818||1494098||2013||7 صفحه PDF||سفارش دهید||دانلود رایگان|
• Application of macroporous resins to endophytic fungal fermentation.
• Established a method to isolate and purify Spiropreussione A from endophytic fungal broth.
• AB-8 resin simultaneously enriched and purified Spiropreussione A.
• One-step resin 2.5-fold enrichment of Spiropreussione A with purity over 90%.
It is attractive to pharmaceutical works to seek useful material from endophytic fungi. Spiropreussione A (SA) which is isolated from endophytic fungus Preussia sp. is a novel anti-tumor product. Since previous preparation method cannot provide enough SA, and considering the large volume of broth and low concentration of the target product, macroporous resins were introduced to separate SA in our study. Four kinds of macroporous resins ADS-8, H103, X-5 and AB-8 were applied for separating SA, and AB-8 was selected as the optimal resin according to its performances through static and dynamic measurements. HPLC was used to analyze SA in all samples. Under optimal conditions, the specific SA adsorption capacity of AB-8 resin was 15.23 mg/g, and the purity increased by 2.5-fold from 35.0% in broth to 90.0% in eluent with 70.0% recovery yield by a one-step treatment. Conclusively, our study achieved the goal of separating and purifying SA in high efficiency, and offered references for further fermentation works.
Journal: Journal of Chromatography B - Volume 938, 1 November 2013, Pages 1–7