Ionic-liquid based dispersive liquid–liquid microextraction followed by high performance liquid chromatographic determination of anti-hypertensives in rat serum

• First report on eco-friendly IL-DLLME of antihypertensives from rat serum.
• Highest extraction recoveries ranging from 92.85 to 98.50%.
• Highest enrichment factors ranging from 4.6 to 4.9

A novel, simple and eco-friendly ionic liquid based dispersive liquid–liquid microextraction followed by HPLC determination of anti-hypertensive drugs viz., eprosartan, valasartan, irbesartan, losartan and telmisartan in rat serum has been developed and validated. Experimental parameters influencing the extraction efficiency, nature and volume of the ionic liquid, dispenser solvent, extraction time and effect of salt were optimized. Under the optimum conditions, the extraction recoveries were between 92.85 and 98.50%. The relative standard deviations of intra-and inter-day accuracy varied between 1.9 and 9.1% (n = 3). The linearity of the proposed method was 0.1–20 μg/mL with coefficients of determination varying between 0.9979 and 0.9992.